• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    The present invention relates to a method for cultivating anti-cancer functional fortified radish, which comprises ocher as a main component, (i) 450 g to 1850 g of sulfur per 1 m 3 of ocher, (ii) standard fertilizer for yellow mud and (iii) 1 m 2 of yellow loess. It is characterized by cultivating the hot radish in the ocher layer made by mixing 1.5 kg. According to the cultivation method according to the present invention, by cultivating the hot radish in the sulfur-treated ocher layer, the hot radish containing a large amount of anticancer substances can be harvested compared to the hot radish grown in the general soil.
    公开号:KR20030025892A
    申请号:KR1020020057600
    申请日:2002-09-23
    公开日:2003-03-29
    发明作者:노치웅；황해준；신정호；강동주；김재호；최혜선
    申请人:경상남도；
    IPC主号:
    专利说明:

    Cultivation method of anticancer functional fortified radish {METHOD FOR CULTIVATING ANTICANCER FUNCTIONAL-ENRICHED YOUNG RADISH}
    [1] The present invention relates to a method of cultivating anti-cancer functional fortified radish, and more particularly, to a method for cultivating anti-cancer functional fortified radish by cultivating it in an ocher layer mixed with sulfur to increase the amount of anticancer substance in the radish.
    [2] In general, methanol extracts of cruciferous vegetables such as radish, heat radish, and cold cultivated in general soil show more than 70% of cancer cell proliferation inhibitory effect against K-562 human blood cancer cells. Although low, it has also been reported to have inhibitory effects (Lee Sun-mi et al., Food Industry and Nutrition, 1 (2), 49-79, 1996). In addition, sulfaraphane ((-)-1-isothiocyanato-4- (methylsulfinyl) butane], an anticancer component, was found in the organic solvent extract of saga broccoli belonging to Cruciferaceae (Zhang et al., 1994), Isothiocyanates found in cruciferous plants are known to be potent inhibitors of carcinogenesis in laboratory animals. Thus, cruciferous vegetables, including yeolmu, are known to contain more anticancer substances than any other kind of vegetables known to date.
    [3] Yeast radish, which belongs to cruciferous plants containing useful anticancer substances, uses young leaves of radish and has been widely used as a summer vegetable in summer, and its demand increases every year, resulting in annual production and annual consumption (周年 消費). ) Is done. Also, it is a health vegetable that is a source of vitamins and minerals, and can be used as an ingredient and main ingredient of kimchi and can be consumed by various recipes.
    [4] Therefore, the present inventors focused on the fact that if the concentration of anticancer substances in yeolmu can be prevented through the ingestion of food and ultimately contribute to the public health, yeolmu containing a large amount of anticancer substances, that is, anticancer functionalities The study was repeated to cultivate fortified radish.
    [5] As a result, the present inventors cultivated yeolmu in the yellow soil layer mixed with sulfur, and completed the present invention by confirming that cultivated yeolmu contained a large amount of anticancer substances compared to yeolmu grown in general soil.
    [6] In addition, the conventional functional reinforcement method is to add the desired functionality mainly in the processing and processing process after crop harvesting, the cultivation method according to the present invention is cultivated iso cyan has an anticancer effect from the first harvest by cultivating heat radish in sulfur-treated loess The significance of the present invention is that the compound can be contained in a large amount.
    [7] Accordingly, it is an object of the present invention to provide a method for cultivating anticancer functional fortified radish containing a large amount of anticancer substance.
    [8] Another object of the present invention is to provide a method of cultivating yeolmu to increase the production of yeolmu containing a large amount of anticancer substances.
    [9] In order to achieve the above object, the anti-cancer functional fortified cultivation method according to the present invention comprises ocher as the main component, (i) sulfur 450 g ~ 1850 g per 1 m 3 of loess, (ii) standard amount of thermal radish and (iii) loess It is characterized by cultivating the hot radish in the loess layer made by mixing 1.3 kg ~ 1.7 kg compost per 1 m 2 .
    [10] In addition, the ocher layer is composed of an upper layer of 1.5 cm to 2.5 cm thick and a lower layer of 5 cm to 7 cm thick, wherein the upper ocher layer is mixed with (i) thermoless fertilization and (ii) 1.5 kg of compost per 1 m 2 of ocher. And the lower loess layer is made by mixing (i) 450 g to 1850 g of sulfur per 1 m 3 of ocher, (ii) standard fertilization of thermal mud and (iii) 1.3 kg to 1.7 kg of compost per 1 m 2 of ocher. It is done.
    [11] Hereinafter, the cultivation method of the present invention will be described in more detail.
    [12] Collect the unoccupied loess and mix it evenly several times (i) 450 g to 1850 g of sulfur per m 3 of loess, (ii) standard fertilizer ratio (iii) and 1.3 kg to 1.7 kg of compost per m 2 of loess. After that, ocher is put in a box of a certain size and sown about 60 granules per box. Cover the soil after sowing and supply enough moisture to prevent the soil from being distracted. After that, depending on the condition of water, watering once or twice a day. After that, when the main leaf is about 7 sheets, harvest.
    [13] Ocher 1 m The amount of sulfur and 450 g ~ 1850 g is suitable per 3, 450 g than when low, the amount of the anti-cancer substance containing the grown young radish not so high as compared to a radish grown in normal soil, the sulfur amount is 1850 If it is higher than g, the radish may not grow enough to harvest the radish.
    [14] In this case, the standard fertilization amount refers to a uniform standard that defines the amount of fertilizer that the crop can harvest the maximum yield when the crop is cultivated, and the standard fertilization amount of yeolmu is N, P, and K, respectively, 3.8 kg / 10a. , 4.4 kg / 10a, 3.9 kg / 10a.
    [15] Compost is a natural organic fertilizer that helps cultivate young radish in addition to chemical fertilizers. It is enough for compost 1.3 kg ~ 1.7 kg per m 2 of loess.
    [16] However, according to the cultivation method as described above, the yield is significantly lower than the yield of yeolmu cultivated in the general soil. This is due to the fact that the roots of young plants are directly in contact with sulfur when they germinate, resulting in poor initial growth.
    [17] In order to solve these problems and to keep the yield of radishes in line with the yield of radishes grown on ordinary soils, the whole 6.5 cm ~ 9.5 cm thick ocher layer 1.5 cm ~ 2.5 cm Separated into a top layer of thickness and a bottom layer of 5 cm to 7 cm thickness, the upper ocher layer mixes (i) thermal standard fertilization and (ii) 1.5 kg of compost per 1 m 2 of ocher, and the lower ocher layer is (i) 1 m of ocher sulfur 450 g ~ 1850 g, per 3 (ii) YR are mixed standard application rate and (iii) ocher 1 m ~ 1.7 kg per kg compost 1.3 second.
    [18] As such, by not treating the upper end of the ocher layer directly touched by the young roots of young radishes with sulfur, the initial growth of the young radishes is vigorous, thereby increasing the total yield of the radishes.
    [19] Through the following examples will be described the present invention in detail. These examples are only for illustrating the present invention in detail, and the scope of the present invention is not limited to these examples.
    [20] Example 1 Cultivation of Yeast Radish (A)
    [21] Collected ocher not grow crops (i) ocher sulfur 455 per 1m 3 g + YR standard application rate (the content of components with N: P: K = 3.8 kg / 10a: 4.4 kg / 10a: 3.9 kg / 10a) + ocher 1 m 2 compost 1.5 kg, (ii) ocher 1m 3 sulfur 909 per g + YR standard fertilizer + ocher 1 m 2 compost 1.5 kg, (iii) ocher 1m 3 sulfur per each 1818 g + YR standard application rate per + ocher 1 m 2 per 1.5 kg compost was evenly mixed several times. After that, three boxes of 0.58 m x 0.38 m x 0.08 m were prepared, and each of the prepared loess of (i), (ii) and (iii) was contained and seeded with about 60 grains per box. After sowing, the soil was covered with soil, and the soil was supplied with sufficient moisture so as not to be disturbed. Thereafter, watering was performed once or twice a day depending on the state of moisture. After that, when the leaves were about 7 sheets, they were harvested.
    [22] Example 2 Cultivation of Yeast Radish (B)
    [23] By separating the hwangtocheung of total 8 cm thickness to planting the young radish as the 2 cm thickness of the top layer and 6 cm thick bottom layer upper hwangtocheung has, except that handle lower hwangtocheung only ocher 1 m of sulfur per 3 1818 g without the Sulfur Was cultivated and harvested thermal radish in the same manner as in Example 1.
    [24] Example 3: Determination of Isothiocyanate-like Compounds in Thermos
    [25] Take 100 g of harvested radish (A), homogenize in DMSO / dimethylformamide / acetonitrile, evaporate the soluble fraction, dissolve in water, add murocinase to add glucosinolate to isothiocyanate Conversion and condensation with 1,2-benzenedithiol to form 1,3-benzenodithiol-2-thione, measured at a wavelength of 365 nm with a spectrophotometer and confirmed by HPLC 20 [Mu] l was loaded into a C 18 column and isocratic elution with 80% methanol to show a retention time peaking at 3.9 minutes. Table 1 shows the results compared with the heat radish grown on the general soil.
    [26] TABLE 1
    [27] processA 365 / gArea of isothiocyanate / g Ocher + sulfur 1818 g / m 3- (iii) of Example 11.305,434,591 Ocher + sulfur 909 g / m 3- (ii) of Example 10.843,171,916 Ocher + sulfur 455 g / m 3- (i) of Example 10.832,887,330 General soil0.692,139,170
    [28] As can be seen from Table 1, the anticancer isothiocyanate-like compound was detected 1.3 ~ 2,5 times higher in the heat radish grown according to the present invention than the heat radish grown in ordinary soil. This experiment shows that there is a compound that can anticancer activity in heat radium itself and the amount is rapidly increased when the soil is treated with sulfur.
    [29] Example 4 Determination of Anticancer Activity Effect of Yeast Radish
    [30] The results of measuring quinone reductase inducing activity with anticancer activity using the hepa lclc cell line (quinone reductase inducing activity) are shown in Tables 2 and 3 below.
    [31] TABLE 2
    [32] processQR derivative specific activity / g 10-fold dilution50-fold dilution Ocher + sulfur 1818 g / m 3- (iii) of Example 15.995.32 General soil4.123.60
    [33] TABLE 3
    [34] divisionOcher + sulfur 1818 g / m 3- (iii) of Example 1Lower Ocher + Sulfur 1818 g / m 3 -Example 2General soil QR active0.2310.1380.070 Indices330197100
    [35] As shown in Table 3, when sulfur was used 1818 g / ㎥ in loess, it showed three times higher activity than general soil, and when sulfur treatment was performed only in the lower part of the loess, it was twice as active as general soil. Was high.
    [36] Example 5: Determination of Saponin-like Compounds in Radish
    [37] In addition, the cultivated heat radish was extracted with 50% ethanol and measured by HPLC under conditions that could detect a saponin-like compound to identify the saponin-like compound in the extract. Saponin standard was found at 41 min residence time under HPLC elution conditions using C18 column, eluting with 1-10 minutes in water, 10-50 minutes in 50% acetonitrile in water and 50-60 minutes in 50% acetonitrile. In comparison with the result, even in the heat radish extract cultivated according to the present invention, a peak was found at the retention time of 41 minutes and the area thereof is shown in Table 4 below.
    [38] TABLE 4
    [39] processArea / g of saponin-like compound Ocher + sulfur 1818 g / m 3- (iii) of Example 110,723,676 Ocher + sulfur 909 g / m 3- (ii) of Example 19,685,955 General soil14,946
    [40] Thus, yeolmu cultivated in sulfur-treated loess contains not only an isocyanate compound having anticancer and blue blood action, but also additionally contains a large amount of saponin compound component extracted from ginseng.
    [41] Example 6: Yield of thermal radish according to cultivation method (A) and cultivation method (B)
    [42] When the main leaves of the heat radish cultivated according to Examples 1 and 2 had about 7 sheets, they were harvested and their respective yields were measured. Table 5 shows the results. When the yield of heat radish harvested from general soil is 100, only 21% of Example 1 according to the cultivation method (A) is 21%, whereas in Example 2 according to the cultivation method (B), the yield of heat radish is 71%. It is up to.
    [43] TABLE 5
    [44] process(Iii) -cultivation method (A) of Example 1Example 2-Cultivation Method (B)General soil Quantity (kg / 10a)644.82230.03161.1 Indices2171100
    [45] As can be seen through Table 5, by dividing the ocher layer into upper and lower parts, only the lower layer is treated with sulfur to cultivate the hot radish, thereby achieving a much higher yield than the yield of the hot radish grown in the sulfur-mixed ocher layer. Therefore, although cultivation of hot radish in the sulfur-blended ocher layer enhances its functionality, it is possible to further improve the hot radish cultivation method, which is difficult to put to practical use due to the extremely low yield of hot radish.
    [46] As described above, according to the cultivation method according to the present invention by cultivating yeolmu in the sulfur-treated ocher layer can be harvested yeolmu containing a large amount of anticancer substances compared to yeolmu cultivated in the general soil, the yield of yeolmu It is expected to be very useful for improving the added value of young radish-producing farmers as well as for improving the health of the nation, and further developing the young radish as a functional kimchi raw material to develop Korea's own export products.
    权利要求:
    Claims (2)
    [1" claim-type="Currently amended] Based on ocher, cultivation of young radish is carried out in the ocher layer consisting of (i) 450 g to 1850 g of sulfur per 1 m 3 of ocher, (ii) standard fertilization of hot radish and (iii) 1.3 kg to 1.7 kg of compost per m 2 of loess. Cultivation method of anticancer functional fortified radish, characterized by the above-mentioned.
    [2" claim-type="Currently amended] 2. The ocher layer of claim 1, wherein the ocher layer comprises an upper layer of 1.5 cm to 2.5 cm thick and a 5 cm to 7 cm thick sublayer, wherein the upper ocher layer comprises (i) thermal aerobic fertilization and (ii) compost per m 2 of loess 1.3. kg to 1.7 kg, wherein the lower loess layer comprises (i) 450 g to 1850 g of sulfur per m 3 of loess, (ii) standard thermal fertilization and (iii) compost per 1 m 2 of loess 1.3 kg to 1.7 kg A method for cultivating anticancer functional strengthening radish, characterized in that made by mixing.
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    同族专利:
    公开号 | 公开日
    KR100454724B1|2004-11-03|
    KR20010099361A|2001-11-09|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    法律状态:
    2001-09-21|Priority to KR1020010058803A
    2001-09-21|Priority to KR1020010058803
    2002-09-23|Application filed by 경상남도
    2003-03-29|Publication of KR20030025892A
    2004-11-03|Application granted
    2004-11-03|Publication of KR100454724B1
    优先权:
    申请号 | 申请日 | 专利标题
    KR1020010058803A|KR20010099361A|2001-09-21|2001-09-21|The methods for young radish culture involving bioactive materials|
    KR1020010058803|2001-09-21|
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